In biacore systems, the surface plasmon resonance spr phenomenon occurs when polarized light, under conditions of total internal reflection, strikes an electrically conducting gold layer at the interface between media of different refractive index. Biacore t200 cytiva, formerly ge healthcare life sciences. The handbook also includes a system description, safety instructions, specifications and a brief description of the spr principle. A distinct mechanism for the abc transporter btucdbtuf. Surface plasmon resonance spr technology is a powerful. Overview of biacore systems and their applications jason. The specificity of a biacore analysis is determined through the nature and. Biacore x100 handbook br100810 edition ac 9 important user information important user information biacore x100 is intended for research use only and should not be used for diagnostic purposes in any clinical or in vitro procedures. Cytiva formerly ge healthcare life sciences 3,909 views. A more detailed description of the parameters and equations is provided in the biacore ge healthcare t100 software handbook br100648, edn. In basic and applied research, clearly defining interaction characteristics ensures that the best antibodies are selected as research tools or therapeutic candidates.
Data are derived from the vant hoff plots and eyring plots shown in figure 6. Extending the throughput of biacore 4000 biosensor to. Quantitative analysis of small moleculenucleic acid. Ge biacore t200 getting started guide ligand analyte. Includes computer loaded with biacore control software. Biacore t100 instrument handbook describes the instrumentation in the biacore t100 system, with instructions for operation, maintenance and troubleshooting. Data from itc experiments are shown in parentheses. Any functionality that is added in optional addon modules is described in separate documentation. These data provide insights into molecular function and disease mechanisms. This getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Biacore t100 gxp handbook describes functionality added with the optional. Curve fitting cannot be applied to plots that have been sorted. The actual sensor surface is shielded within a protective sheet. Its technology is often used to measure not only binding affinities, but kinetic rate constants and.
Handbook of surface plasmon resonance richard b m schasfoort, richard schasfoort, arnoud marquart, peter schuck, erk t. Biacore t100 instrument handbook guides you through operation and maintenance of the biacore t100 system. Reagents and samples are loaded into a reagent rack and a microplate, or into a combined sample and reagent rack. It gives accurate and rapid results with a minimum of operator involvement, thus speeding up the time to produce results and increasing productivity in the lab. Biacore sensor surface handbook br100571 ac 11 introduction 1 1. Although it is useful to experiment with different software packages, biaevaluation current version 4. Biacore t200 instrument handbook, the instruction manual for operating the biacore t200 instrument. Thermodynamic parameters of the interactions studied using biacore t100. In case of the biacore instruments this are the sensor chips. From chemistry analyzers that analyze urine, blood, and plasma, to bioanalyzers that use microfluidic technology.
Sensor surface handbook br100571 biacore concentration analysis handbook br100512 used in biacore t200, biacore t100, biacore 4000 and biacore s51. From biacore t100 to biacore t200 2 5 6 8 10 lod 0. The interaction being studied occurs on the surface of the chip. The biaevaluation software of the biacore 4000 biosensor only allows analysis of the data generated using the predefined assays provided by the manufacturer ge healthcare and cannot be utilized to analyze data generated using novel assays. Used in biacore x100, biacore 3000, biacore c, biacore 2000, biacore, biacore x, and biacore j. Its reliability and success is built on biacores unique expertise in three cornerstone technologies. In the case of classical injections singleanalyte injections, data sets were analyzed with biacore t100 evaluation software, biaevaluation 1. Antibodies and other proteins can be fully characterized within days. Biacore t200 instrument handbook guides you through operation and maintenance of the biacore t200 system.
Biacore surface matrix effects on the binding kinetics and. With exceptional sensitivity, this system enables interaction analysis with greater precision and confidence than ever before. These analyses can be performed using a multicycle approach many samples against one ligand or when different ligands are immobilized, or alternatively, using singlecycle kinetics fast runs without regeneration. Biacore t200 getting started 28984098 edition ab 5 biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Reference corrected spr binding curves red, monitored on a surface of covalently immobilized caii, for various concentrations of 4carboxybenzenesulfonamide 2fold dilution series from 100 nm 25. Legal information regarding software licensing and details for acquiring additional licenses. The data generated from biacore t100 experiments run over several temperatures are also shown in graphic form in figure 7. The options that the ge biacore software provides are not useful, export curves just export a bitmap with a very bad quality by the way and export result table does not export the sensogram data.
It has flexible tools for customizing your data reporting and processing. A waste bottle and a bottle containing water are placed on the waste and water tray. Lab analyzers can provide info on the size, the quantity of cells and the purity of samples. Page 129 the closeness of fit is reported for linear fitting as the correlation coefficient r and for 4parameter fitting as the chisquared value see section 9. The reagent rack andor microplate are placed on a rack tray. The ge biacore t200 evaluation software can be operated with a few clicks, and enables kinetic and affinity evaluations of interactions. Biacore epitope scouting analysis of antibodies are carried out on biacore 3000 for epitope mapping in order to identify mabs that recognize distinct separate epitopes on a single antigen. The biacore 2000 by biacore ab is a biomolecular analyzer that utilizes surface plasmon resonance spr technology to monitor biomolecular interactions in real time.
Biacore assay handbook 29019400 edition aa 5 introduction 1 1 introduction 1. The chemistry in the biacore the chemical first reaction is the reaction of c convected by the flow with d which is bounded at the wall. Evaluation of the biacore 2000 instrument for screening of low molecular weight affinity ligands title swedish abstract an important stage in the development of new affinity media for protein purification is the screening of potential affinity ligands. The k a was determined by the kinexa direct method where solutions without cmdextran added containing 449 pm mab binding site and 655 pm ag were followed to equilibrium for each replicate measurement. This note provides guidelines and recommendations for using this function to integrate biacore. The equation for the reaction products formation b k is the ratio of the constant of reverse and direct reaction is. This limitation is another challenge associated with the biacore 4000 biosensor. Christina burtsoff asp marketing manager ge healthcare. Lab analyzers can be used to analyze samples in a variety of settings. The biacore 4000 is ideal for laboratories that focus on drug discovery and antibody analysis with the ability to perform 60 hours of unattended operation, the biacore 4000 reduces labor by the researcher and increases productivity. Biacore t200 getting started guide, which in conjunction with the getting started reagent kit and cm5 sensor chip, provides users a selfguided tutorial through the basic steps of a basic biacore experiment performed using aminecoupling chemistry. Biacore is a life science products company, based in sweden. Biacore t200 software offers a range of tools for confident and reliable kinetic analyses.
First step for biacore insight evaluation software. Certificate grade cm5 chips are coated with capture antibody goat antimouse igg fc for mouse mab s, goat antihuman igg fc for chimeric, humanized or. Latest software updates and software notes software upgrade packages and additional licences software wizard templates to help speed up assay optimization and routine analyses handbooks covering system operationmaintenance and qflex kits system brochures and application notes. Free mab binding site was quantified as a function of time after mixing ag with mab. It is used to find the mass concentration of biomolecules that are adjacent to a. The spr machines use sensor surfaces to have a defined matrix on which the measurements are done. For the biacore t100 user, data processing can be performed automatically using the biacore t100 evaluation software, which is much more convenient for new users. A food safety tester or pathogen analyzer or clinical analyzer. Surface plasmon resonance spr plays a dominant role in realtime interaction sensing of biomolecular binding events and with the biosensor field expanding more applications are being found. Biacore is a fully automated system for biomolecular interaction analysis. Dataset of the binding kinetic rate constants of anti. Biacore spr biosensors can be used for a wide variety of assays, including specificity, active concentration measurement, kinetics, and affinity and thermodynamic parameters. Ge healthcare protein interaction analysis biacore sensor. Biacore 3000 is the highest performance research system available for labelfree studies of biomolecular binding and an ideal system for all research laboratories, providing high quality data on the kinetic and affinity parameters for a given biomolecular interaction.
High performance, versatility and ease of use with biacore t100. Evaluation of the biacore 2000 instrument for screening of. Check out the new biacore insight evaluation software platform tailored for biacore 8k, biacore t200, and biacore s200. Depending on the application one of the sensor chips is used. This edition of the biacore x100 handbook applies to systems running software version 2. Assay design and setup for flu vaccine analysis duration. Biacore t100 software handbook br100648 edition ae. The instrument also features a rack hotel in which up to 10 rack trays can be loaded with automatic rack exchange. Spr is a noninvasive optical measuring technique that does not require the labelling of interacting components. Biacores spr technology has been designed to investigate the functional nature of binding events. This handbook describes biacore t100 control software and evaluation software, version 2. Biacore x100 system from ge healthcare life sciences. Binding of 4carboxybenzenesulfonamide to carbonic anhydrase ii caii, monitored on a biacore t100 instrument. The biacore t100 data were doublereferenced using reference flow cell 1 and the subtraction of a preceding buffer blank with biaevaluation v.
The handbook, together with the associated reagent kit, will take you through the basic steps in a biacore experiment. Recovering samples from biacore 3000 for mass spectrometry. Biacore t200 is a versatile system for labelfree interaction analysis offering solutions all the way from basic research, through drug discovery to process development and quality control. Sensor chip technology the biacore sensor chip is at the heart of the technology.